RNA Methodologies A Laboratory Guide for Isolation and Characterization

The isolation of biologically competent and chemically stable RNA is central to molecular biology. As there is currently a need to unify components of RNA characterization, this text presents RNA isolation strategies and a collection of protocols in a coherent start-to-finish format. It is written for anyone capable of conducting basic research, and addresses basic skills involved in nucleic acid research as well as some of the more sophisticated protocols. A collection of tried and tested laboratory protocols for the isolation and characterization of mammalian RNA, this book focuses on the study of RNA as a parameter of gene expression. "RNA Methodologies: A Laboratory Guide for Isolation and Characterization" emphasizes how these methodologies can be utilized in the study of transcriptional and post-transcriptional regulation of eukaryotic genes. The chapters work together to tell the RNA story, each including clear take-home lessons. The liberal incorporation of flow charts, tables and graphs facilitate learning and assist in the planning phases of a project.;The text studies cellular regulation using RNA as a parameter of gene expression, offers RNA isolation strategies and explains propert handling, storage and manipulation of RNA.