Practical Protein Chemistry A Handbook

Protein chemistry is a wide field embracing the analysis, synthesis, sequencing and separation of peptides, proteins and individual amino acids. As research work in this subject area increases, an authoritative survey of proven techniques is required.;Practical Protein Chemistry is just such a book, designed primarily to be a laboratory handbook for research workers involved with proteins, it will also be of use to undergraduates and their tutors.;Each chapter commences with an authoritative introduction to the subject with an account of the practical problems involved. The authors then provide full practical details of methods which they have found to work well, and references to each technique so that they can be researched in the literature with ease.;Space necessarily limits the methodologies described - essentially the purification of proteins and peptides, separation of mixtures, determination, sequencing, amino acid analysis, and chemical and enzymatic degradation are all covered. Physico- chemical techniques are largely omitted - but mass spectrometry, X-ray crystallography and electron microscopy which are all important tools for structural studies are included in detail. The final chapter concerns itself with the prediction of peptide and protein structure which may be made from sequencing data.