Introduction to Fluorescence Microscopy - Microscopy Handbooks

This handbook covers three main areas. Firstly it deals with the physical principles of the phenomenon of fluorescence and fluorescence microscopy (including transmitted versus incident illumination), and gives a detailed description of the various parts of the microscope: lenses, filters, and light sources. It goes on to discuss the main applications of fluorescence microscopy, for example immunofluorescence, microfluoremetry (including absorbance and fluorescence photometry), and fluorescence photomicrography. The final chapter, on trouble-shooting, will enable the reader to solve the most common problems in fluorescence microscopy. Each chapter is followed by an up-to-date list of references to review articles, books, and specialist publications.;Throughout the book, the authors have taken care to emphasize the general aspects of fluorescence microscopy as well as illustrating their description with examples of typical microscopes and optical components.;Readership: cell biologists, clinicians, researchers and technicians, and undergraduates in the life and medical sciences interested in obtaining a thorough grounding in fluorescence microscopy.