Publisher's Synopsis
Transmission electron microscopy is one of the most important and popular forms of microscopy used in biomedical research. "Electron Microscopy - A Practical Approach" was published in 1987, and contained techniques relating specifically to nucleic acids and proteins. This volume provides protocols for a range of procedures relating to the electron microscopy of tissues, cells and their components with the emphasis on biomedical applications. The fundamental methods for specimen preparation, tissue fixation, embedding and sectioning are covered and are followed by the more specialist techniques of freeze-substitution, immunogold labelling, autoradiography and enzyme histochemistry.;Although the technical details in the book relate mainly to tissues and cells, many of the procedures can be adapted for isolated organelles and membrane fractions, viral particles, macromolecular assemblies and large proteins. This is a broadly based compilation of the most important specimen preparation techniques and their applications. It is designed for researchers and advanced students working in almost every biomedical discipline, plus technicians in electron microscopy units.
