Publisher's Synopsis
Lack of suitable tissue culture methods hampers the ability to determine the viability and infectivity of certain United States Environmental Protection Agency (USEPA) contaminant candidate list (CCL) pathogens isolated in source and drinking water supplies. In addition to gathering occurrence data in water supplies, these infectivity assays assist water utilities and USEPA in determining the risk of infection should these pathogens be detected by nucleic acid methods (eg: polymerase chain reaction (PCR) and its variant, quantitative real-time PCR) in public water supplies.... The primary objective for this work was to develop an infectivity assay for human norovirus. (NoV) using in vitro cell culture techniques.
